发明名称 |
EXPRESSION SYSTEMS FOR FUNCTIONAL MEMBRANE POLYPEPTIDES |
摘要 |
<p>Expression systems and methods for the expression of functional membrane polypeptides such as human cytochrome b5 are provided. The systems include recombinant expression vectors capable of expressing soluble fusion proteins that include a solubilizing agent, a linker, and a membrane polypeptide, as well as one or more cleavers, e.g. proteases, capable of cleaving the linker to release the membrane polypeptide. When the fusion protein is expressed, the linker is cleaved by the cleaver to allow association of the membrane polypeptide with a membrane.</p> |
申请公布号 |
WO2009064954(A1) |
申请公布日期 |
2009.05.22 |
申请号 |
WO2008US83503 |
申请日期 |
2008.11.14 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION;FOX, BRIAN, G.;SOBRADO, PABLO |
发明人 |
FOX, BRIAN, G.;SOBRADO, PABLO |
分类号 |
C12N9/02 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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