METHOD TO MEASURE AMINO ACID CONCENTRATIONS

摘要

The instant invention provides a method of determining amino acid concentration in a sample, by performing an aminoacyl-tRNA synthetase charging reaction at equilibrium which comprises a cognate aminoacyl-tRNA synthetase, a cognate tRNA, a fixed quantity of radiolabeled target amino acid, and the sample comprising the target amino acid. The method further quantifies the signal emitted from the resultant aminoacylated cognate tRNA by a scintillation proximity assay, and compares the signal against a calibration curve to reveal the concentration of the target amino acid in the sample.