METHOD FOR THE DESIGN AND ENGINEERING OF OLIGONUCLEOTIDES

摘要

The present invention relates to a method for the design of oligonucleotides suitable for PCR, Real time PCR, microarrays and, more generally for DNA and RNA amplification and/or detection, said method allowing a quick and simultaneous computation of more parameters, and computer programs capable of carrying out the method of the invention. The present invention discloses a method and a computer program carrying out said method integrating, all in one, a high functionality, and a number of parameters that are not measured together in the state of the art, for more than one oligonucleotide spamming over a single input sequence starting from sequences in FASTA format and giving a few input data such as threshold temperatures, oligonucleotide length and sampling frequencies.