发明名称 Method For Preparing Genome Library, And Genome Library Prepared By The Method
摘要 There is provided a first method of preparing a genome library in which PCR is carried out with the use of genomic DNA of target biological species per se or fragment thereof as a direct template and with the use of random primer or one type of primer of specified sequence so as to effect genome amplification, thereby obtaining a genuine library. There is further provided a second method of preparing a genome library in which genome of target biological species is pretreated and thereafter PCR is carried out with the use of one type of primer of inherent sequence so as to effect genome amplification, thereby obtaining a genome library. In both of the methods, a genome library can be prepared conveniently from a minute amount of sample.
申请公布号 US2009131275(A1) 申请公布日期 2009.05.21
申请号 US20040591555 申请日期 2004.03.16
申请人 SHIMAMOTO NOBUO;NAKAYAMA HIDEKI;ARAMAKI HIRONORI 发明人 SHIMAMOTO NOBUO;NAKAYAMA HIDEKI;ARAMAKI HIRONORI
分类号 C12N15/09;C40B40/08;C12N15/10;C12Q1/68;C40B50/06 主分类号 C12N15/09
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