METHOD OF SYNTHESYSING NUCLEOSIDE-5'-TRIPHOSPHATES, LABELLED WITH RADIOACTIVE ISOTOPES OF PHOSPHOROUS IN ALPHA-POSITION

摘要

FIELD: biology. ^ SUBSTANCE: present invention relates to biotechnology, more specifically to obtaining nucleoside-5'-triphosphates, labelled with phosphorous-32 (phosphorous-33) in the alpha-position, and can be used for analysis in molecular biology, genetics and medical biochemistry. The method is realised through treatment of labelled nucleosidephosphate in a buffer solution with a mixture of deoxyribonucleoside monophosphate kinase of bacteriophage T5 and pyruvate kinase with subsequent chromatographic purification of the target product. ^ EFFECT: simple method of obtaining nucleoside-5'-triphosphates and stable output of the target product. ^ 4 ex