发明名称 |
Method of expanding undifferentiated hemopoietic stem cells |
摘要 |
A method of expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) obtaining the undifferentiated hemopoietic stem cells; and (b) culturing the undifferentiated hemopoietic stem cells in a medium containing a stromal cell conditioned medium, the stromal cell conditioned medium being derived from a stationary phase plug-flow bioreactor in which a three dimensional stromal cell culture has been established under continuous flow of a culture medium on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding the undifferentiated hemopoietic stem cells.
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申请公布号 |
US7534609(B2) |
申请公布日期 |
2009.05.19 |
申请号 |
US20050102625 |
申请日期 |
2005.04.11 |
申请人 |
PLURISTEM LIFE SYSTEMS INC. |
发明人 |
MERCHAV SHOSHANA;MERETSKI SHAI |
分类号 |
C12M3/00;A61F2/00;A61K35/12;A61K35/14;A61P7/00;A61P43/00;C12M3/04;C12N5/00;C12N5/02;C12N5/078;C12N11/08;C12R1/91 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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