| 摘要 |
Treatment of blood and blood fractions with a mixture of methylene blue and crystal violet ("Double-Dye") has been found to eliminate a variety of bacteria, virus and parasites without appreciably damaging blood cells or proteins. A 4-log reduction in Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Yersinia enterocolitica, Pseudomonas aeruginosa, Pseudomonasfluorescens and Serratia marcescens was observed. Complete kills of vesicular stomatitis virus, encephalomyocarditis virus, bovine diarrheal virus and porcine parvovirus were achieved by adding sufficient ascorbate to largely convert the two dyes into their leuco forms. Finally, a PVA filtration method is used to remove the dyes before use of the disinfected blood or blood component. Thus, the Double-Dye-ascorbate-PVA method is an effective, low-cost way to eliminate pathogens from whole blood, platelets, plasma or other blood fractions. |
