METHOD OF QUANTIFYING TRANSIENT INTERACTIONS BETWEEN PROTEINS

摘要

A method of detecting and quantifying a transient interaction between two different proteins is described. One potentially interacting protein is transformed into a fusion protein with a binder protein, the other one is modified with a substrate specific for the binder protein to give a substrate protein. The modified proteins are allowed to interact, and any interaction is trapped by reaction of the substrate with the binder protein. The reaction product of the substrate with the binder protein is detected and quantified, and the result used to quantify the interaction between the two potentially interacting proteins.