NON-INVASIVE REAL-TIME IN VIVO BIOLUMINESCENCE IMAGING OF LOCAL CA 2+ DYNAMICS IN LIVING ORGANISMS
摘要
A method for bioluminescence imaging in an animal is provided. The method comprises providing a whole animal containing a transcriptionally active nucleic acid sequence encoding a Ca2+-sensitive polypeptide, which comprises or consists of a chemiluminescent protein linked to a fluorescent protein; and monitoring photons emitted by the Ca2+-sensitive polypeptide. The Ca2+-sensitive polypeptide comprises or consists of aequorin protein covalently linked to a YFP (yellow fluorescent protein) or RFP (red fluorescent protein), and the link between the two proteins functions to allow luminescence by energy transfer between the two proteins. The photons are monitored from deep tissues of the animal.
申请公布号
WO2008104830(A3)
申请公布日期
2009.05.07
申请号
WO2007IB03218
申请日期
2007.08.24
申请人
INSTITUT PASTEUR;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;BRULET, PHILIPPE;ROGERS, KELLY, LEE;CURIE, THOMAS