METHOD AND APPARATUS FOR DETECTING FLUORESCENCE EMITTED BY PARTICLE-BOUND FLUOROPHORES CONFINED BY PARTICLE TRAPS
摘要
<p>A method of detecting a fluorescence signal emitted by fluorophores bound to particles confined in a particle trap, includes an objective lens having a focal plane, which is normally the focal plane for incident collimated light. The particle trap is typically located in the focal plane, and a beam of excitation light is directed via the objective lens onto the confined particles in the trap. The excitation light is in the form of a divergent beam coming to focus at a plane displaced from the focal plane. The divergent beam has a spot diameter at the focal plane determined by the divergence of the beam. The fluorescent light emitted by the fluorophores is detected with a confocal detector.</p>
申请公布号
WO2009055903(A1)
申请公布日期
2009.05.07
申请号
WO2008CA01673
申请日期
2008.09.25
申请人
NATIONAL RESEARCH COUNCIL OF CANADA;BOUDREAU, DENIS;GRAVEL, JEAN-FRANCOIS;VOISIN, BENOIT;LE DROGOFF, BORIS;VERES, TEODOR