RADIOLABELED MONOCLONAL ANTIBODIES, METHODS OF PREPARATION USING TCEP, AND USES THEREOF

摘要

This application discloses methods for producing radiolabeled IgM or IgA monoclonal antibodies using tris(2-carboxyethyl) phosphine hydrochloride (TCEP) to reduce disulfide bonds to generate sulfhydryl (-SH) groups and treating the monoclonal antibody with a radioisotope that binds to -SH groups, radiolabeled antibodies produced by the methods, monoclonal anti-melanin IgM antibodies containing about 42 to about 45 -SH groups, and methods of treating and imaging cancer, such an melanoma, and infections using the radiolabeled antibodies.