发明名称 |
DEVICE AND METHOD FOR HIGH THROUGHPUT SCREENING OF CRYSTALLIZATION CONDITIONS IN A VAPOR DIFFUSION ENVIRONMENT |
摘要 |
A high-density high-throughput microplate and methods for simultaneously screening a plurality of protein crystallization solutions and for producing diffraction quality protein crystals in a vapor-diffusion environment are disclosed. The microplate has defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 µl, and wherein the paired chambers have a vapor channel, therein providing vapor exchange between the side-by-side paired chambers. The microplate further includes a membrane to seal the surface of the microplate. The microplate is adapted to receive a crystallization solution in one of the side-by-side paired chambers and a protein solution in the other of the side-by-side paired chambers, wherein the protein solution and the crystallization solution interact via a vapor diffusion process, which enables the formation of protein crystals within the chamber that contains the protein solution.
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申请公布号 |
CA2704188(A1) |
申请公布日期 |
2009.05.07 |
申请号 |
CA20082704188 |
申请日期 |
2008.04.25 |
申请人 |
JANSSEN PHARMACEUTICA N.V. |
发明人 |
LEWANDOWSKI, FRANCIS A.;SPURLINO, JOHN C.;KWASNOSKI, JOSEPH D. |
分类号 |
B01L3/00;B01L3/06;C30B7/00;C30B29/58 |
主分类号 |
B01L3/00 |
代理机构 |
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主权项 |
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