| 摘要 |
PROBLEM TO BE SOLVED: To provide a preferred site for adding a cysteine residue to the growth hormone supergene family comprising greater than 20 structurally related cytokine and growth factors, or for introducing cysteine substitution, and a protein and protein derivatives produced thereby. SOLUTION: A general method for preparing a site-specific biologically active conjugate of the growth hormone supergene family proteins such asβ-interferon or the like is provided. This method comprises a step in which a cysteine residue is added to a non-essential region of this protein, or a step in which a non-essential amino acid in the protein is substituted with a cysteine residue by using site-specific mutagenesis, and then a step in which the protein is connected to a cysteine reactive polymer through the added cysteine residue or another type cysteine reactive part is connected with a covalent bond. COPYRIGHT: (C)2009,JPO&INPIT |
