发明名称 |
RNA EXTRACTION METHOD, RNA EXTRACTION REAGENT, AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS |
摘要 |
A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.
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申请公布号 |
US2009111114(A1) |
申请公布日期 |
2009.04.30 |
申请号 |
US20080251375 |
申请日期 |
2008.10.14 |
申请人 |
YAMASHITA YOSHIHIRO;SAKURAI TOSHINARI;KUNO NORIHITO;UCHIDA KENKO;YOKOBAYASHI TOSHIAKI |
发明人 |
YAMASHITA YOSHIHIRO;SAKURAI TOSHINARI;KUNO NORIHITO;UCHIDA KENKO;YOKOBAYASHI TOSHIAKI |
分类号 |
C12N15/09;C12Q1/68;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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