摘要 |
A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula (I) wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase. |
申请人 |
APPLERA CORPORATION;XI, LARRY, L.;PROSEN, DENNIS, E.;NOVIKOV, ALEXANDER, A. |
发明人 |
XI, LARRY, L.;PROSEN, DENNIS, E.;NOVIKOV, ALEXANDER, A. |