METHOD FOR THE SYNTHESIS OF NUCLEIC ACID BY USING FRAGMENTED NUCLEIC ACIDS AND N-PRIMER

摘要

The present invention relates to a method for the synthesis of nucleic acid based on RNA fragmentation and N-primer which is prepared by using a fragmented RNA as a template, a method for constructing a cDNA library, a cDNA library constructed by said method, and a kit comprising N-primer for the synthesis of nucleic acid. According to the present invention, N-primer which may have the same complementarity for all regions of mRNA and various kinds of nucleic acids including mRNAs that are randomly fragmented by an artificial method are used for the synthesis of cDNAs or strands of complementary nucleic acids for every region. Thus, an overlap can be avoided and all kinds of cDNAs originating from mRNA or base sequences of the nucleic aids that are complementary to mRNA can be obtained.