ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME

摘要

It is intended to provide a DNA polymerase suitable for a specific isothermal amplification method and an isothermal amplification method using the same. An amplification reaction of a target nucleic acid sequence in a nucleic acid sample is performed at an isothermal temperature using a first primer shown in the following (X) in the presence of a DNA polymerase comprising the following protein (a) or (b). By using the DNA polymerase, the amplification reaction using the primer can be performed in a shorter time than conventional. (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 23, (b) a protein comprising an amino acid sequence represented by SEQ ID NO: 25 (X) a primer containing a sequence (Ac') hybridized to a sequence (A) in a region at the 3' end of the target nucleic acid sequence in a region at the 3' end and further containing a sequence (B') hybridized to a complementary sequence (Bc) to a sequence (B) present at the 5' side of the sequence (A) in the target nucleic acid sequence at the 5' side of the sequence (Ac').