NANO-LIPID-BASED CARRIERS FOR TARGETED DELIVERY OF VIRAL VECTORS AND PROCESS FOR ITS PRODUCTION

摘要

The present invention is related with lipidic nanovesicles which carry genetic material, particularly in the form of recombinant viruses (viral vectors), composed by neutral lipids and/or neutral and negative lipids, a viral vector, and a ligand responsible for the targeting of the nanoparticles to the target cells that will be transduced by the carried viral vectors, in order to guarantee the specific delivery of genes to target cells, as well as their process of production. The process of production of the nanoparticles includes essentially three main stages: 1) association of the viral vectors to the lipidic nanonvesicles; 2) conjugation of a hydrophilic polymer to the surface of the nanovesicles; 3) conjugation of a ligand to the distal end of the hydrophilic polymer. Following this method of preparation, the invention presents adequate characteristics for the delivery of genes to a specific group of cells after administration, namely intravenous administration, independently of the normal tropism of the vector associated to the nanovesicles. This approach allows the treatment of pathologies involving cells not accessible by a local application, and thus minimize the secondary effects that may emerge as a consequence of the unspecific interaction of the viral vectors with other cells, as well as the innate immune response triggered against these vectors. Accordingly, the invention is to be used in the pharmaceutical field, namely for the treatment of neoplastic, inflammatory, cardiovascular and neurodegenerative disease, among others, namely involging gene delivery mediated by viral vectors.