METHOD FOR EXTRACELLULAR PRODUCTION OF TARGET PROTEINS USING ARTJ, OSMY OR FKPA

摘要

A new promoter form expressing and secreting a target protein is provided to produce pure target protein by extracellularly expressing the target protein. Gene constructs comprise: a secretion promoting motif gene selected among a group of a coding gene of arginine transporter subunit(ArtJ), a coding gene of hyperosmotically inducible periplasmic protein(OsmY) and a coding gene of peptidyl-prolyl cis-trans isomerase(FkpA) and a gene coding a target protein. The gene constructs are characterized in intracelluarly expressing in a fusion form of the secretion promoting motif selected and the target protein and extracelluarly secreting. Method of extracellular secretion and production of the target protein comprises: a step of culturing recombinant microorganism containing the gene constructs to extracellularly secret the target protein; and a step of collecting the target protein in culture media.