摘要 |
The present invention relates to chemical sensing and in particular to a method for detecting an analyte in a sample of whole blood. The method comprise, in summary, the steps of: exposing the sample to a transducer having a tethered reagent; introducing a labelled reagent; irradiating the sample with a series of pulses of electromagnetic radiation at a wavelength of 600 run or above; and transducing and detecting the electrical signal and the time delay between each pulse. The label on the labelled reagent absorbs the electromagnetic radiation at a level which is at least equal to the absorption of the sample of whole blood at the wavelength of the electromagnetic radiation used. |