SINGLE-READOUT MULTIPLEXING OF METAGENES

摘要

The present invention is related to a method of analyzing a biological sample comprising the following steps: (a) providing a first group of probes comprising at least a first probe capable of specifically binding a first biological molecule belonging to a first group of biological molecules and a second probe capable of specifically binding a second biological different from the first biological molecule, but also belonging to the first group of biological molecules, (b) reacting said first probe and said second probe with said sample such as to form a binding complex of the probes with the respective biological molecule, (c) providing a first label yielding a detectable signal upon binding of the first probe to the first biological molecule or upon binding of the second probe to the second biological molecule, wherein a combined detectable first signal results when one or both of first and second biological molecule are present in the sample; and (d) reacting said first label to detect binding of probes with the respective biological molecule. The biological molecules may be gene products of housekeeping genes in order to provide improved internal controls for gene expression analysis assays.