METHODS OF SCREENING FOR COMPOUNDS HAVING ANTI-INFLAMMATORY ACTIVITY

摘要

A method of screening for compounds out of the group consisting of proteins, peptides, peptidomimetics, antibodies or small organic molecules that increase the activity of the protein Rac1 by virtue of their binding to the extracellular portion of this protein, the method comprising the steps of: a) contacting a confluent layer of cultured endothelial cells grown on porous membranes with at least one of the test compounds alone or together with a stimulant of vascular permeability, b) measuring the endothelial permeability with a suitable agent, which can be colorimetrically detected.