发明名称 |
IN VITRO SELECTION OF APTAMER BEACONS |
摘要 |
Provided herein are methods of selecting aptamer beacons in vitro using single-stranded nucleic acid species comprising a fluorphore and a random region of N nucleotides. The single-stranded nucleid acid species are annealed to a capture oligonucleotide comprising an F1 quenching moiety. Aptamer beacons are eluted using a target ligand or analyte to interact with the capture single-stranded nucleic acid species to release it from the capture oligonucleotide. Those selected single-stranded nucleic acid species comprise aptamer beacons. Also provided are the aptamer beacons so selected and methods of detecting a ligand in solution using selected aptamer beacons. |
申请公布号 |
WO2005019430(A3) |
申请公布日期 |
2009.04.02 |
申请号 |
WO2004US27284 |
申请日期 |
2004.08.23 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
ELLINGTON, ANDREW;MANJULA, RAJENDRAN |
分类号 |
C07H21/04;C12N;C12P19/34;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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