摘要 |
In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) culturing a plurality of immature conifer somatic embryos for a first incubation period in, or on, a first development medium that comprises less than 0.1% trehalose; and (b) culturing the plurality of immature conifer somatic embryos treated in accordance with step (a) for a second incubation period in, or on, a second development medium that comprises at least 0.1% trehalose.
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