发明名称 |
Gene, enthaltend eine für Hydroxynitrillyase codierende DNA-Sequenz, rekombinante Proteine mit Hydroxynitrillyase-Aktivität und deren Verwendung |
摘要 |
Gene (I) containing a DNA sequence that encodes a hydroxynitrile lyase (HNL) is new. It is prepared using a combination of primers based on the 5' and 3' regions of the MDL1 gene of Prunus amygdalus and one of the mdl1-5 genes of P. serotina, by standard amplification of DNA from organisms that contain HNL-encoding genes, then cloning. Independent claims are also included for: (1) recombinant protein (II) produced by heterologous expression of the new HNL gene sequences from P. amygdalus in a host cell; (2) fusion protein (FP) with HNL activity prepared using (I) and a signal sequence for secretory expression in a host cell; and (3) method for preparing (S)- or (R)-cyanohydrins (II) by reacting an aliphatic or (hetero)aromatic aldehyde or ketone with a cyanide donor in presence of (II) or FP, in organic, aqueous or two-phase systems, or in an emulsion. |
申请公布号 |
DE50114716(D1) |
申请公布日期 |
2009.04.02 |
申请号 |
DE2001514716 |
申请日期 |
2001.12.18 |
申请人 |
DSM FINE CHEMICALS AUSTRIA NFG GMBH & CO KG |
发明人 |
SCHWAB, HELMUT;GLIEDER, ANTON;KRATKY, CHRISTOPH;DREVENY, INGRID;POECHLAUER, PETER;SKRANC, WOLFGANG;MAYRHOFER, HERBERT;WIRTH, IRMA;NEUHOFER, RUDOLF;BONA, RUDOLFO |
分类号 |
C12N15/09;C12N15/60;C07K19/00;C12N9/04;C12N9/88;C12N15/52;C12P13/00 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|