DNA APTAMER BINDING TO RETINOL BINDING PROTEIN 4 WITH SPECIFICITY AND PRODUCTION METHOD THEREOF

摘要

A DNA aptamer, which is specifically bound to a rentinol-binding protein(RBP4), is provided to effectively diagnose the type 2 diabetes. A DNA aptamer which can be specifically combined with a rentinol-binding protein(RBP4) has one base sequence among SEQ ID NO:1 to 10. A composition for diagnosing the type 2 diabetes comprises the DNA aptamer specifically combined with a rentinol-binding protein. The composition for diagnosing the type 2 diabetes measures a level of blood RBP4. The RBP4 indicates a biomarker of the type 2 diabetes. A method for preparing the DNA aptamer comprises the following steps of: reacting the RBP4 and magnetic beads to a borate buffer solution to induce covalent bonds; mixing the RBP4-magnetic beads to a DNA pool having 30-50 numbers of bases in a center and a primer region for PCR(polymerase chain reaction) in an end so as to induce bonding at a room temperature.