Binding of inorganic phosphate to a phosphate binding protein can result in changes to the stacking of appropriately positioned chromophores, thereby resulting in a detectable change. The invention provides a phosphate-binding protein that undergoes a conformational change from an initial conformation to a final conformation upon binding of phosphate, wherein the protein carries a first label and a second label, and wherein the first and second labels are arranged such that they exhibit molecular stacking that is perturbed on changing from one conformation to the other. Preferred labels are rhodamines.
申请公布号
EP2041164(A2)
申请公布日期
2009.04.01
申请号
EP20060779250
申请日期
2006.08.31
申请人
THE MEDICAL RESEARCH COUNCIL
发明人
CORRIE, JOHN, EDGAR, THOMAS;WEBB, MARTIN, RONALD;OKOH, MICHAEL, PRINCE