METHOD FOR MANUFACTURING OF SINGLE-CHAIN ANTIBODY AGAINST ZEARALENONE USING RECOMBINANT METHYLOTROPHIC YEAST

摘要

A method for preparing single-chain antibody against zearalenone using recombinant methylotrophic yeast is provided to produce stabilized antibody with excellent expression rates, solubility and antigen affinity. A method for preparing single-chain antibody against zearalenone using recombinant methylotrophic yeast comprises the following steps of: amplifying single-chain antibody against zearalenone using a PCR(polymerase chain reaction) method; cloning the amplified single-chain antibody against zearalenone to an expression vector and verifying existence of the cloning; transforming the cloned expression vector to methylotrophic yeast and selecting recombinant yeast; and expressing single-chain antibody against zearalenone among selected recombinant yeast.