RECOMBINANT PLASMIDE DNA pVar15-HIV-LTR, CARRYING CLONED FRAGMENT GENOME HIV OF 1 TYPE FROM CONSERVATIVE SITE OF 5-LTR SEQUENCE, RECOMBINANT PLASMIDE DNA pBluKSM-HIV-LTR mod CARRYING CLONED MODIFIED FRAGMENT OF SAME SITE GENOME HIV OF 1 TYPE, TEST SET FOR QUANTITATIVE EXPRESS IDENTIFICATION OF GENOME HIV-1 OF ANY TYPE IN ASSAY AND METHOD WITH ITS USE

摘要

FIELD: medicine; molecular biology. ^ SUBSTANCE: invention concerns molecular biology, genetic engineering and can be used in medicine. Quantitative express identification of the HIV-1 genome in assay is detected with the help of oligonucleotide primers, complementary to a conservative site 5'-LtR-part of the HIV-1 genome, fluorescently marked probes and the RNA-modified fragment of a conservative site of 5'LTR area of the HIV-1 genome as the internal quantitative standard. The analysis is performed in real time in "the closed test tube" format by means of the calibration curves received with the help plasmides pVarl5-HIV-LTR and pBluSK-HIV-LTR mod, containing the native and modified kdnk-fragment virus genome from conservative 5'-LTR-pocledovatelnocti accordingly. Plasmide pVar15-HTV-LTR it is received on the basis of the pVar15 plasmides. The pBluSK-HIV-LTR mod plasmides is received on the basis of pBluKSM plasmide. ^ EFFECT: possibility of determination of HIV-1 presence in assay irrespective of type of a virus, a combination of types and presence of related kinds. ^ 4 cl, 10 dwg, 9 ex