USES OF IMMUNOLOGICALLY MODIFIED SCAFFOLD FOR TISSUE PREVASCULARIZATION AND CELL TRANSPLANTATION

摘要

This invention provides method of making and using of a porous 3 dimensional cyclic RGD peptide-modified alginate scaffold that can be loaded with different cell types and/or growth factors for implantation at sites of tissue damage to promote tissue regeneration. The cyclic RGD peptide promotes vascular formation of the host tissue, cell binding and survival of seeded cells. In vitro, cyclic RGD peptide promotes cell survival more efficiently than linear RGD peptide, which results in improved live cell numbers after prolonged culture. Scaffolds with growth factors but without cells can be implanted to create a vascular bed in which cells are transplanted at a later time point, for example pancreatic islets.