BIOREACTOR, CELL CULTURE METHOD, AND SUBSTANCE PRODUCTION METHOD

摘要

The present invention achieves a smaller difference between a dissolved oxygen concentration in an upper part of a culture tank and a dissolved oxygen concentration in a lower part of the culture tank. The present invention comprises: a culture tank; a sparger means arranged in a lower part of the culture tank; and multiple impellers being arranged in multiple stages in a vertical direction of the culture tank and having a larger mass transfer capacity coefficient KLa per unit number of revolutions in an upper stage than in a lower stage.