Substrate and method for producing the substrate

摘要

To enable measurements having high S/N ratios, by suppressing non specific adsorption. A substrate that includes: a base material; and chelators which are three dimensionally covalently bound to the base material at a density of 7.8×10 15 /mm 3 or greater and 4.5×10 17 /mm 3 or less, or two dimensionally covalently bound to the base material at a planar density of 2.0×10 11 /mm 2 or greater and 1.0×10 13 /mm 2 or less, is used. Metal ions are coordinately bound to the chelators, and a bioactive substance having histidine tags are coordinately bound to the metal ions. A blocking agent having ligand sets are coordinately bound to the metal ions to which the bioactive substance is not coordinately bound.