MULTIPLEX FLOW IMMUNOASSAYS WITH MAGNETIC PARTICLES AS SOLIDPHASE

摘要

Heterogeneous assays for different analytes in a single biological sample ar e performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles vary in size to encompass distinct size ranges that are distinguishable by flow cytometry, the particles in each size range carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other size ranges. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment.