METHODS OF NUCLEIC ACID AMPLIFICATION ANALYSIS

摘要

A method for quantifying nucleic acid sequences in amplification or reverse transcription reactions, involving the use of real-time detection (RTD) polymerase chain reaction (PCR) systems. The invention includes formulating mathematical models, based on kinetic descriptions of PCR for different RTD systems, and development of application algorithms for these models to quantitatively analyze RTD-PCR experiments. The invention also includes quantitative analysis of the effects of non-specific (secondary reactions) amplification on the efficiency of specific target amplification. The invention also includes quantitative analysis of multiple specific concurrent amplifications (multiplex reactions) including internal control relative quantification experiments.