| 摘要 |
<P>PROBLEM TO BE SOLVED: To rapidly and comprehensively detect the expression of a cell surface protein genetic cluster of mammals. <P>SOLUTION: This method for designing a probe for the cell surface protein genetic cluster of the mammals comprises designing the probe according to following standards: (1) a step of selecting a transmembrane region or a GPI anchor modification site of the cell surface protein, (2) a step of determining the probe of 50-70 mers when the base sequence of 50-70 mers containing the selected transmembrane region or GPI anchor modification site has 40-60% GC content, (3) a step of designing the probe having the 40-60% GC content according to the order of a specific process when the cell surface protein has the transmembrane region and the base sequence of 50-70 mers containing the transmembrane region can not have the 40-60% GC content and (4) a step of designing the probe having the 40-60% GC content according to the order of the specific process when the cell surface protein has the GPI anchor modification site and the base sequence of 50-70 mers containing the GPI anchor modification site can not have the 40-60% GC content. <P>COPYRIGHT: (C)2009,JPO&INPIT |
