摘要 |
Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. |
申请人 |
KYOTO UNIVERSITY;CANON KABUSHIKI KAISHA;YAMAUCHI, FUMIO;YOSHIMURA, KIMIHIRO;KUGE, KATSUAKI;YANO, TETSUYA;SHIRAKAWA, MASAHIRO;AOYAMA, YASUHIRO;SANDO, SHINSUKE |
发明人 |
YAMAUCHI, FUMIO;YOSHIMURA, KIMIHIRO;KUGE, KATSUAKI;YANO, TETSUYA;SHIRAKAWA, MASAHIRO;AOYAMA, YASUHIRO;SANDO, SHINSUKE |