Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50 -fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
申请公布号
WO2008141824(A3)
申请公布日期
2009.03.12
申请号
WO2008EP04090
申请日期
2008.05.21
申请人
BAXTER INTERNATIONAL INC.;BAXTER HEALTHCARE S.A.;MATTHIESSEN, PETER;ROMEDER-FINGER, STEFAN;TURECEK, PETER;SCHWARZ, HANS-PETER