ANALYTE DETECTION VIA BINDING MOLECULE-ASSOCIATED ENZYME ASSAY

摘要

Methods, kits, and compositions for the detection and quantification of an analyte are provided. Typically, in the methods a complex is formed between an analyte-specific binding agent and an analyte. The analyte-specific binding agents comprise a binding molecule and an enzyme possessing an activity that produces a PCR template indicative of the presence of the analyte. Amplification and detection of the PCR template yields a sensitive and quantitative measurement of analyte concentration.