METHODS OF GLYCOSYLATION AND BIOCONJUGATION

摘要

The instant invention provides isolated catalytic domains from a polypeptidyl-a- N-acetylgalactosaminyltransferase (pp-GalNAc-T). The invention provides methods for engineering a glycoprotein from a biological substrate, and methods for glycosylating a biological substrate for use in glycoconjugation. Also included in the invention are diagnostic and therapeutic uses. The invention further provides in vitro folding methods for pp-GalNAc-T.