| 摘要 |
<p>The present invention relates to the field of biotechnology, and in particular to obtaining secondary metabolites, via induction, such as bromelain from plants of the genus Ananas spp., previously established in vitro. The subject of the invention is the developments of an innovative process for extracting bromelain from the natural tissues of the pineapple (Ananas comosus (L) Merr) plant that have been established and induced in vitro with a higher proteolytic activity (200 to 600%) - and in high concentrations - as compared with the protein generated naturally in the plant and currently extracted using conventional methodologies. In this process, use is made of pineapple seedlings established and induced in vitro, which are macerated in a phosphate buffer for the purposes of their extraction. Later, they are subjected to filtering, centrifugation, freezing and lyophilization processes in order thus to obtain a crude bromelain extract characterized by high protein yields and specific activity.</p> |
