Biosensor capable of simultaneous detection of substrate binding and reaction product

摘要

An object of the present invention is to provide a biosensor that can detect binding of a compound with a functional protein and then assay a reaction product derived from the activity of the functional protein again. The present invention provides a biosensor for detecting a test molecule (5) specifically binding to a physiologically active substance (6), which comprises; (a) a first reaction region (10) on which the physiologically active substance (6) has been immobilized for performing a binding reaction between the physiologically active substance (6) and the test molecule (5) and a physiologically active reaction caused by the physiologically active substance (6), and (b) a second reaction region (11) on which a molecule that specifically binds to a reaction product (7) resulting from the physiologically active reaction has been immobilized for performing a binding reaction between the reaction product (7) and the molecule (8) that specifically binds to the reaction product (7), in the same area; and an assay region (3) for detecting changes in the binding reaction in the first reaction region (10) and in the binding reaction in the second reaction region (11). The assay region (3) may be a waveguide.