发明名称 |
DNA polymorphism identity determination using flow cytometry |
摘要 |
DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand. |
申请公布号 |
EP2034031(A1) |
申请公布日期 |
2009.03.11 |
申请号 |
EP20080104631 |
申请日期 |
1998.10.28 |
申请人 |
LOS ALAMOS NATIONAL SECURITY, LLC |
发明人 |
NOLAN, JOHN P.;WHITE, P. SCOTT;CAI, HONG |
分类号 |
C12N15/00;C12Q1/68;C07H21/02;C07H21/04;C12N15/09 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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