PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF RNA POLYMERASE alphaSUBUNIT AS A FUSION EXPRESSION PARTNER
摘要
Provided is a method for manufacturing a recombinant protein, which can be used for protein medicine and industrial production by using a RNA polymerase alpha subunit as a fusion partner. A method for manufacturing a recombinant protein comprises the following steps of: manufacturing an expression vector by connecting a heterologous protein gene to a RpoA(RNA polymerase alpha subunit) gene as a fusion partner; manufacturing a transformant by employing the expression vector to a host cell; and inducing expression of the recombinant protein by cultivating the transformant. The RpoA gene is written as SEQ ID NO:45.
申请公布号
KR20090025484(A)
申请公布日期
2009.03.11
申请号
KR20070090371
申请日期
2007.09.06
申请人
KOREA UNIVERSITY INDUSTRIAL & ACADEMIC COLLABORATION FOUNDATION
发明人
LEE, JEE WON;PARK, JIN SEUNG;HAN, KYUNG YEON;SONG, JONG AM;SEO, HYUK SEONG;AHN, KEUM YOUNG;LEE, JONG HWAN;LEE, EUN JEONG;KWON, SOO JEONG