发明名称 |
PRODUCTION OF ADENO-ASSOCIATED VIRUS IN INSECT CELLS |
摘要 |
A method of producing an adeno-associated virus (AAV) in an insect cell comprising (i) providing at least one insect cell-compatible vector comprisi ng a first nucleotide sequence comprising at least one AAV ITR nucleotide sequence, a second nucleotide sequence containing an open reading frame encoding AAV VP1, VP2, and VP3 capsid proteins, a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence, and a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence, (ii) introducing the at least one insect cell-compatible vector into an insect cell, and (iii) maintaining the insect cell under conditions such that AAV is produced. Also provided are recombinant AAV made in accordance with the method, insect cell - compatible vectors, and insect cells comprising nucleotide sequences for production of AAV in an insect cell.
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申请公布号 |
CA2467959(C) |
申请公布日期 |
2009.03.10 |
申请号 |
CA20022467959 |
申请日期 |
2002.11.08 |
申请人 |
GOVERNMENT OF THE UNITED STATES OF AMERICA, DEPARTMENT OF HEALTH AND HUM AN |
发明人 |
URABE, MASASHI;KOTIN, ROBERT M.;DING, CHUAN-TIAN |
分类号 |
C12N15/864;C07K14/015;C12N5/10;C12N7/00;C12N7/01;C12N15/35;C12N15/866 |
主分类号 |
C12N15/864 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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