| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: method involves sampling with further sample degassing. Further, if luminescent sea bacterium Photobacterium phosphoreum B-17 677f is added to test and reference samples, then sample mineralisation level is defined and NaCl is added to the test sample to reach total mineralisation level of 30 g/l. Reference samples are prepared. Further, 0.01 solution of NaCl is added to test and reference samples to reach pH 7.5. Then luminescent sea bacteria Photobacterium phosphoreum B - 17 677f or recombinant luminescent bacteria Esherichia coli K12 TGI with luminescent system genes of Photobacterium leognathi are added to test and reference samples. Further the luminescence level is measure, and toxicity index is calculated by the formula T=((Ir-It)/Ir)*100%, where Ir is luminescence intensity in reference sample, It is luminescence intensity in test sample. With T50% it is considered highly toxic. ^ EFFECT: improved accuracy of method. ^ 2 tbl, 1 ex |
