General methods and strains of bacteria are described, that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.
申请公布号
WO2009025690(A2)
申请公布日期
2009.02.26
申请号
WO2008US06553
申请日期
2008.05.22
申请人
NATURE TECHNOLOGY CORPORATION;WILLIAMS, JAMES, A.
发明人
WILLIAMS, JAMES, A.;HODGSON, CLAGUE, P.;CARNES, AARON, E.