| 摘要 |
A method and kits for assaying a sample of a human or mammalian bodily fluid to simultaneously determine whether one or more of a plurality of drugs and/or metabolites thereof are present in said sample and optionally to perform a semi-quantitative assay for said drug or drugs, comprising: (a) incubating the sample in a competitive assay with a plurality of drug conjugates and a plurality of primary antibodies that bind to the drugs whose presence and optionally whose amount is to be determined, wherein either the plurality of drugs or the plurality of primary antibodies is coupled to microparticles comprising magnetically responsive material, the particles being divided into subsets of particles, each distinguishable from the others by one or more differentiation parameters and by the drug to which they are coupled; (b) incubating the product of step (a) with a liquid medium comprising one or more labeled ligands for the primary antibodies or drug conjugates; (c) magnetically separating microparticles in all of said groups from said liquid medium; and defining said liquid medium as a first liquid medium; (d) resuspending said microparticles separated therefrom in a second liquid medium; and (e) analyzing said microparticles in said second liquid medium by flow cytometry and identifying drugs present in the sample, and optionally calculating a semi-quantitative result for drugs found to be present.
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