| 摘要 |
Described herein are platforms and consumables for performing gene/siRNA/protein/peptide delivery screens in high throughput, including an automation-compatible transfection methodology that can be used to gain entry of oligonucleotides, proteins, peptides and other non-permeable molecules (e.g., some small organic compounds) into cells. Electrical stimulation is provided to cells, e.g., neurons or myocytes, in culture through application of pulsed fields directly to the cells in culture. The plate design is used in conjunction with a custom-designed circuit that allows the user to select pulse type, duration, etc.
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