FINDING PAIRED ISOTOPE GROUPS

摘要

A technique for finding paired isotope groups of peptides, metabolic materials, or other materials is executed without having to identify features. Any suitable isotopic labeling methods, such as SILAC or ICAT, can be used. The technique can identify isotope pairs by pairing heavy and light labeled peptides based on mono-isotopes. The technique searches for isotope groups that have retention time and mass/charge within given tolerances, adjustable by users. Multiple label sites are supported as well as reverse-labeling to inhibit or reduce biases. Multiple replicates can be merged into a composite image.