METHOD FOR PREPARATION OF RECOMBINANT DNA

摘要

[PROBLEMS] To provide a DNA recombination method which is simplified and has improved efficiency. [MEANS FOR SOLVING PROBLEMS] Disclosed is a method for preparing recombinant DNA by inserting a DNA fragment of interest into vector DNA, which comprises the step of carrying out the following reactions (a) and (b) substantially simultaneously in the same reaction site: (a) a reaction for cleaving both of a site which is located within the vector DNA and into which the DNA fragment of interest is to be inserted and DNA containing the DNA fragment of interest simultaneously in the presence of a restriction enzyme whose DNA recognition site and DNA cleavage site are different from each other; and (b) a reaction for inserting the DNA fragment of interest into the vector DNA in the presence of a DNA ligase.